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electroporation  (Bio-Rad)


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    Bio-Rad electroporation
    Electroporation, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1924 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/electroporation/product/Bio-Rad
    Average 96 stars, based on 1924 article reviews
    electroporation - by Bioz Stars, 2026-03
    96/100 stars

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    Generation of Vero cell line expressing TMUV replicon. (A) The schematic flowchart delineates the key steps required for the establishment of a stable replicon-harboring cell line. (B) Vero cells were electroporated with viral replicon genomic RNA (10 μg) and BSD was supplemented into the medium 2 days later. Gluc activity was quantified in cell culture supernatants at indicated time points. (C) At 30 days <t>post-electroporation,</t> the intracellular total RNA was extracted and RT-qPCR assay was conducted to quantify viral RNA levels. Data represents the mean ± SD ( n = 3). *** p < 0.001. Statistical significance was assessed by two-tailed t -test. LOD represents limit of detection.
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    Generation of Vero cell line expressing TMUV replicon. (A) The schematic flowchart delineates the key steps required for the establishment of a stable replicon-harboring cell line. (B) Vero cells were electroporated with viral replicon genomic RNA (10 μg) and BSD was supplemented into the medium 2 days later. Gluc activity was quantified in cell culture supernatants at indicated time points. (C) At 30 days post-electroporation, the intracellular total RNA was extracted and RT-qPCR assay was conducted to quantify viral RNA levels. Data represents the mean ± SD ( n = 3). *** p < 0.001. Statistical significance was assessed by two-tailed t -test. LOD represents limit of detection.

    Journal: Poultry Science

    Article Title: Establishment of a stable replicon cell line of Tembusu virus (TMUV) for high-throughput antiviral screening

    doi: 10.1016/j.psj.2025.106109

    Figure Lengend Snippet: Generation of Vero cell line expressing TMUV replicon. (A) The schematic flowchart delineates the key steps required for the establishment of a stable replicon-harboring cell line. (B) Vero cells were electroporated with viral replicon genomic RNA (10 μg) and BSD was supplemented into the medium 2 days later. Gluc activity was quantified in cell culture supernatants at indicated time points. (C) At 30 days post-electroporation, the intracellular total RNA was extracted and RT-qPCR assay was conducted to quantify viral RNA levels. Data represents the mean ± SD ( n = 3). *** p < 0.001. Statistical significance was assessed by two-tailed t -test. LOD represents limit of detection.

    Article Snippet: Single electrical pulse was given with the Gene Pulser Xcell Electroporation Systems (Bio-Rad) with setting of 270 V at 950 microfarads.

    Techniques: Expressing, Activity Assay, Cell Culture, Electroporation, Quantitative RT-PCR, Two Tailed Test